cd36 deficient  (Jackson Laboratory)

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 reduces systemic and lung homogenate ROS and inflammatory mediators. (A) Study design. (B) Bar graphs and dot plots represent the relative expression of Igf1 mRNA in lung tissue of CD36 +/+ and (F) CD36 ‐/‐ mice. (C) Mean plasma MDA levels of CD36 +/+ and (G) CD36 ‐/‐ mice, expressed as bar graphs and dot plots. (D) Bar graphs and dot plots represent the relative expression of Nox2 mRNA in lung tissue of CD36 +/+ and (H) CD36 ‐/‐ mice. (E) Bar graphs and dot plots of the total leucocytes recruitment in lung tissue by MPO assay of CD36 +/+ and (I) CD36 ‐/‐ mice. (J) Bar graphs and dot plots represent the mononuclear cells count per mm 2 of photomicrographs of lung tissue of CD36 +/+ and (K) CD36 ‐/‐ . (L) Representative photomicrographs of lungs after staining with hematoxylin‐eosin (scale bar: 100 μm). Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 6–11 per group for CD36 +/+ and n = 6 per group for CD36 ‐/‐ .

Article Snippet: CD36‐deficient (CD36 −/− ) mice and wild‐type littermate controls on a C57Bl/6 J background were obtained from Jackson Laboratories (Bar Harbor, Maine, USA) and bred following established protocols.

Techniques: Expressing, Clinical Proteomics, MPO Assay, Staining

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 reduces lung NLRP3 inflammasome and pro‐inflammatory cytokines and chemokines. Bar graphs and dot plots represent the relative expression of (A) Nlrp3 , (B) Il1β , and (C) Il18 mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ mice. (D) Mean IL‐1β levels in lung homogenates of CD36 +/+ mice, expressed as bar graph and dot plot. Bar graphs and dot plots represent the relative expression of (E) Nfκb1 , (F) Nfκb2 , (G) RelA , (H) RelB , (I) Tnf , (J) Il6 , (K) Ccl2 , (L) Atx , (M) Cxcl1 , (N) Siglecf , and (O) Cd11c mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ . Data are mean ± SEM. * p < 0.05, ** p < 0.01, and ***p < 0.001, as assessed by an unpaired t test. n = 11 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Article Snippet: CD36‐deficient (CD36 −/− ) mice and wild‐type littermate controls on a C57Bl/6 J background were obtained from Jackson Laboratories (Bar Harbor, Maine, USA) and bred following established protocols.

Techniques: Expressing

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 decreases arachidonic acid metabolites‐PGE 2 . PGE 2 levels in lung homogenates of (A) CD36 +/+ and (B) CD36 ‐/‐ mice, represented as bar graphs. Bar graphs and dot plots represent the relative expression of Ptges mRNA in lung tissue of (C) CD36 +/+ and (D) CD36 ‐/‐ mice. (E) Bar graph and dot plot represent the relative expression of Cox2 mRNA in lung tissue of CD36 +/+ . Relative expression of (F) Ptger2 , (G) Ptger4 , and (H) Ptger3 mRNA in lung tissue of CD36 +/+ mice, expressed as bar graphs and dot plots. Bar graphs and dot plots represent the relative expression of (I) Ptger2 and (J) Ptger4 mRNA in lung tissue of CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Article Snippet: CD36‐deficient (CD36 −/− ) mice and wild‐type littermate controls on a C57Bl/6 J background were obtained from Jackson Laboratories (Bar Harbor, Maine, USA) and bred following established protocols.

Techniques: Expressing

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 decreases arachidonic acid metabolites‐LTB 4 . (A) Bar graph and dot plot represent the LTB 4 levels in lung homogenates of CD36 +/+ and (B) CD36 ‐/‐ mice. (C) Relative expression of Alox5 mRNA in lung tissue of CD36 +/+ and (D) CD36 ‐/‐ mice. Bar graph and dot plot represent the relative expression of (E) Alox12 , (F) Alox15 and (G) Alox5ap mRNA in lung tissue of CD36 +/+ mice. (H) Bar graph and dot plot of the relative expression of Ltc4s mRNA in lung tissue of CD36 +/+ . (I) Bar graph and dot plot represent the relative expression of Ltb4r1 mRNA in lung tissue of CD36 +/+ and (J) CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Article Snippet: CD36‐deficient (CD36 −/− ) mice and wild‐type littermate controls on a C57Bl/6 J background were obtained from Jackson Laboratories (Bar Harbor, Maine, USA) and bred following established protocols.

Techniques: Expressing

Journal: Cell metabolism

Article Title: NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement Across Capillaries and into Cardiomyocytes

doi: 10.1016/j.cmet.2018.03.017

Figure Lengend Snippet: NanoSIMS analyses of 2H− ions in the heart after administering an intravenous injection of 200 µl of 2H-TRLs to 4-month-old female wild-type (Cd36+/+) and Cd36−/− mice. 1.5 or 2 min after the injection, hearts were harvested and sections were prepared for NanoSIMS. Differences in quantitative NanoSIMS data were analyzed with a Student’s t test with Welch’s correction. Four mice were used in this experiment, one for each time point.

Article Snippet: Cd36 -deficient mice ( Cd36 −/− ) ( Febbraio et al., 1999 ) and wild-type mice were obtained from The Jackson Laboratory (strain C57BL/6).

Techniques: Injection